论文名称： Effect of protein molecular weight on the mass transfer in protein mixing
作者： Asad， Ahmed*; Chai， Chuan*; Wu， JiangTao
来源出版物： Science China: Physics， Mechanics And Astronomy
年卷期页： 2012,55(3):470-476
收录类型： SCI:913CW;WOS:000301850100017和EI
论文简介： The mixing of protein solutions with that of precipitating agents is very important in protein crystallization experiments.In this work,the interferometry images were recorded during the mixing of two proteins with different molecular weights:lysozyme of ~14.6 kDa,trypsin of ~23.3 kDa and pepsin of ~34.8 kDa were placed in a Mach-Zehnder interferometer.The protein molecular weight dependence on the competition of the transport process and kinetics at the interface was studied.The concentration profiles of protein solutions were calculated to analyze the mass transfer during the mixing process.It was observed that the mass transfer process is more efficient during the mixing of proteins with higher molecular weights.In addition,the more rapid concentration changes above the interface suggest that convection may dominate the diffusion.The phenomenon of convection is higher in the protein solutions with higher molecular weight.
原文链接： Effect of protein molecular weight on the mass transfer in protein mixing